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| Time | Flow (ml/min) | Percent solvent A | Percent solvent B |
| 00:00 | 0.150 | 15.0 | 85.0 |
| 05:00 | 0.150 | 70.0 | 30.0 |
| 15:00 | 0.150 | 70.0 | 30.0 |
| 16:00 | 0.300 | 98.0 | 2.0 |
| 22:00 | 0.300 | 98.0 | 2.0 |
| 23:00 | 0.300 | 15.0 | 85.0 |
| 24:00 | 0.300 | 15.0 | 85.0 |
| 25:00 | 0.400 | 15.0 | 85.0 |
| 30:00 | 0.400 | 15.0 | 85.0 |
