(a) Changes in the fura-2 ratio in vector-introduced (Control, upper), TRPA1a-introduced (middle) and TRPA1a with TRPA1b-introduced (lower) DRG neurons from TRPA1KO mice. Black bars indicate agonist application periods. Responses to TRPA1 alone (blue), TRPV1 alone (red) and TRPV1 with TRPA1 (green) are shown. (b) AITC responses (normalized to the ionomycin responses) were compared. Data represent mean±s.e.m. *P<0.05, **P<0.01, Control n=298; TRPA1a n=312; TRPA1a+TRPA1b n=298, one-way ANOVA with Bonferroni’s post hoc analysis.