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. 2018 Mar 23;9(22):15847–15859. doi: 10.18632/oncotarget.24627

Figure 5. Gpx2 siRNA transfection and ROS signals in BC31 cells.

Figure 5

(A) mRNA expression level of Gpx2 in BC31 cells was confirmed by qRT-PCR 2 days after transfection with two different Gpx2-targeting and negative control (NC) siRNAs. Mean ± SD; ****p<0.0001. (B) Proliferation rate of BC31 cells treated with Gpx2-targeting and NC siRNAs at 5 days after siRNA transfection. Mean ± SD; ****p<0.0001. (C) Guava® apoptosis analysis of BC31 cells after siRNA transfection shows that Gpx2 knock-down induced apoptosis. Mean; ****p<0.0001. (D) DCFH-DA assay was used to quantify intracellular ROS levels after knock-down of Gpx2 by siRNA in BC31 cells. Mean ± SD; ***p<0.001. (E) Western blotting analyses at 5 days after siRNA transfection of BC31 cells. The expression of Gpx2 was reduced, but that of cdc25B, cyclin D1, cyclin B, caspase 7, and caspase 3 were not changed. β-actin was used as internal loading control.