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. 2018 Mar 23;9(22):16028–16042. doi: 10.18632/oncotarget.24678

Figure 1. Effect of ATO on miR-182-5p expression in S1 GBM cells.

Figure 1

(A) Dose-dependent suppression of miR-182-5p expression by ATO (n=4). (B) The melting curves of miR-182-5p and U6 snRNA during the qRT-PCR. (C) The Venn diagram involved four databases that predict putative mRNA targeted by miR-182-5p. The number of each colored circle was the amount of predicted mRNAs. There were 327 predicted mRNAs repeatedly presented in these four databases. (D) Flow cytometric analysis of ATO-induced oxidative stress. (E) NAC rescued ATO-mediated suppression of miR-182 expression. (F) Reporter gene assay results showed that ATO suppressed miR-182 targeting of the 3’UTR of the SESN2 gene. (G) Suppression of ATO-mediated induction of SESN2 by NAC. (H) The results of M TT assay showed that transduction with a miR-182 mimic enhanced the cytotoxic effects of ATO. *: p<0.05.