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. 2018 Mar 23;9(22):16028–16042. doi: 10.18632/oncotarget.24678

Figure 2. MiR-182-5p suppressed the expression of SESN2 and TP53INP1.

Figure 2

(A) Sequence complementary between miR-182-5p and 3’-UTR of SESN2 and TP53INP1. (B) Confirmation of miR-182-5p mimic transduction into GBM cells by qRT-PCR. (C) SESN2 mRNA and TP53INP1 mRNA expression was inhibited following transduction of the miR-182-5p mimic. (D) SESN2 and TP53INP1 protein expression were inhibited following transduction of the miR-182-5p mimic. (E) and (F) Reporter gene assay results demonstrating that the miR-182-5p mimic targets the 3’UTRs of SESN2 mRNA and TP53INP1 mRNA. (G) Tetracycline-induced miR-182-5p expression in H1299 cells transduced with a tet-on-miRNA system. (H) Suppression of SENS2 protein expression following tet-on induction of miR-182-5p expression. (I) Suppression of ATO induced SENS2 expression, but not HO-1 expression, following tet-on induction of miR-182-5p expression. *: p<0.05.