Figure 1. Cisplatin increases ADAM17-dependent AREG release in ovarian carcinoma cell lines.

After 48 h of cisplatin treatment with the indicated concentrations, cells were trypsinized, counted and lyzed. Optical densities (ODs) of ADAM17 and AREG levels in lysates and AREG amounts in supernatants were measured by sandwich ELISA, and the calculated concentration values were normalized to the total cell number. Cisplatin treatment increased ADAM17-protein amounts (left panel) and AREG levels (center panel) in cell lysates and AREG release into culture supernatants (right panel) in Igrov-1 cells and A2780 cells. Skov-3 cells did not respond to cisplatin treatment. Data from three to five independent experiments per cell line are presented as mean + SEM. Stars indicate significant differences compared to untreated cells (0 μM). Friedman test; P = significance, ^* (p<0.05); ^** (p<0.01).