Figure 2. Dabrafenib and AZ628 effects on ERK activity in WT RAF-expressing HEK293T cells.

HEK293T cells were transiently transfected or co-transfected with WT BRAF, WT CRAF, or WT BRAF/WT CRAF expression vectors. 48 h post-transfection, cells were treated for 2 h with DMSO, Dabrafenib (2.5 μM), or AZ628 (2.5 μM), then lysed and subjected to Western blot analysis for the indicated proteins (A) Effects of Dabrafenib and AZ628 on CRAF S338 phosphorylation in CRAF-expressing HEK293T cells (B).