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. 2018 Mar 25;11(1):84–90. doi: 10.3400/avd.oa.17-00086

Fig. 2 Effect of pyridone 6 on the activity of STAT3, MMPs, and TGF-β in human AAA tissue culture. Effect of pyridone 6 on STAT3 activity was evaluated by immunoblotting for activated (phosphorylated) STAT3 (P-STAT3) and total STAT3 after incubation of the AAA tissue with the indicated concentration of pyridone 6 for 48 h. (A) Representative images of immunoblotting for P-STAT3 and STAT3 are shown, and GAPDH was used as the internal control. (B) Expression levels of P-STAT3 normalized to that of GAPDH are represented. (C) Expression levels of STAT3 normalized to that of GAPDH are represented. (D) Secretions of MMP-9 and -2 were evaluated by gelatin zymography of the conditioned media. After collecting the basal secretion for 48 h, AAA tissue culture was treated with or without 100 ng/mL IL-6 and the indicated concentrations of pyridone 6. (E) Basal secretions of TGF-β family are shown in the conditioned media after 48 h of culture. (F) The effect of 10 µM pyridone 6 on the secretions of TGF-β family of cytokines is represented. Data are expressed as means±standard errors. The sample without pyridone 6 treatment was assigned a value of 1. * p<0.05.

Fig. 2 Effect of pyridone 6 on the activity of STAT3, MMPs, and TGF-β in human AAA tissue culture. Effect of pyridone 6 on STAT3 activity was evaluated by immunoblotting for activated (phosphorylated) STAT3 (P-STAT3) and total STAT3 after incubation of the AAA tissue with the indicated concentration of pyridone 6 for 48 h. (A) Representative images of immunoblotting for P-STAT3 and STAT3 are shown, and GAPDH was used as the internal control. (B) Expression levels of P-STAT3 normalized to that of GAPDH are represented. (C) Expression levels of STAT3 normalized to that of GAPDH are represented. (D) Secretions of MMP-9 and -2 were evaluated by gelatin zymography of the conditioned media. After collecting the basal secretion for 48 h, AAA tissue culture was treated with or without 100 ng/mL IL-6 and the indicated concentrations of pyridone 6. (E) Basal secretions of TGF-β family are shown in the conditioned media after 48 h of culture. (F) The effect of 10 µM pyridone 6 on the secretions of TGF-β family of cytokines is represented. Data are expressed as means±standard errors. The sample without pyridone 6 treatment was assigned a value of 1. * p<0.05.