FIG. 2.

Droplet trapping chip for prolonged observation and imaging, including differently shaped chambers of variable depth (a). The incubated and imaged droplets encapsulate growing microorganisms (Streptomyces sp. and red fluorescent E. coli) labeled with distinct fluorescent markers (b). Multilevel structure for the fabrication of chips with facile optical fiber insertion and optimal droplet detection at varying channel heights [(c) and (d), (e)]. Calibration curves for detected light comparing a single-layer channel of 50 μm height with the presented multilevel structure. Each data point represents the average for more than 1000 droplets per condition; the error bars represent one standard deviation, with the inset detailing lower concentrations (f). Multimedia view: https://doi.org/10.1063/1.5013325.1 Download video file ^{(878.3KB, avi)} DOI: 10.1063/1.5013325.1