Figure 4.

Inhibition of the mTOR pathway induces upregulation of Par-4 and Foxo3a. A, BT-474 cells were transduced with lentivirus expressing a control shRNA or one of two shRNAs targeting mTOR. Four days later, cells were treated with vehicle or Lapatinib for 3 days and levels of Par-4 and components of the mTOR pathway were measured by Western blotting. B and C, BT-474, MCF-7, and SKBR3 cells were treated with Torin1 for 2 days and levels of Foxo3a were measured by qRT-PCR (B) or Western blot (C). Significance was determined by Student’s t-test and data are presented as mean plus SD. **, p<0.01; ***, p<0.001.