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. Author manuscript; available in PMC: 2019 Apr 3.
Published in final edited form as: Chem Commun (Camb). 2018 Apr 3;54(28):3532–3535. doi: 10.1039/C8CC01424H

Figure 4.

Figure 4

Effect of oleic acid on the SAA secondary structure and amyloid formation. (A) Non-denaturing PAGE of SAA in the absence (gray) and in the presence of OA (blue). SAA (0.5 mg/ml) was treated with 5 mM OA as described in ESI Methods. (B) Far-UV CD spectra show that OA induced helical folding in SAA at 25 °C, from the largely unfolded to 40% α -helical conformation. (C) Time course of amyloid formation by SAA in the absence and in the presence of OA monitored by ThT fluorescence. (D) Far-UV CD spectrum of fibrillar OA-treated or lipid-free SAA after 72 h incubation at 37 °C. Insert: TEM of OA-treated SAA after incubation.