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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Mol Cancer Res. 2018 Feb 16;16(4):720–727. doi: 10.1158/1541-7786.MCR-17-0480

Fig. 4. The Androgen Receptor contributes to spontaneous bone metastasis in breast cancer.

Fig. 4

a) Immunoblot shows AR expression levels in parental, lung variant and bone variant MDA-MB-231 cells. Error bars represent SEM. n=3; *P<0.04. b) Schematic of the experiment to determine the propensity of AR expressing bone variant MDA-MB-231 cells to metastasize to the lung or bone (left). Representative images of GFP-stained primary breast tumor, lung and bone metastases (right) derived from the bone variant MDA-MB-231 cells inoculated into the mouse mammary gland. c) The bar graph shows the number of animals with bone and lung metastases in the groups harboring tumors in which AR expression was suppressed with 2 different shRNAs versus control shRNA. n=8; *P=0.048. d) The bar graph shows the number of animals with bone and lung metastases in the groups treated with vehicle or enzalutamide. n=8; **P<0.009 (bottom).