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Age decreased hit rate compared to young intact males (A). GDX rats did not exhibit sexual behaviors, whereas testosterone replacement in GDX rats increased the hit rate (B). Middle-aged rats did not ejaculate during the testing period (C). Testosterone replacement in GDX rats increased ejaculation frequency (D). Fisher’s LSD post hoc analyses: *** p ≤ 0.05 vs. young intact males; # p ≤ 0.05 vs. young GDX.
