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. Author manuscript; available in PMC: 2019 Apr 2.
Published in final edited form as: Curr Biol. 2018 Mar 22;28(7):1124–1131.e3. doi: 10.1016/j.cub.2018.02.039

Figure 4. Aged granulosa cells have an impaired ability to generate TZPs.

Figure 4

(A) Left: Phalloidin-stained TZPs in oocytes from 3-month and 13-month females. Right: The number and density of TZPs and oocyte diameter were determined from confocal images. (B) FLIP was performed on COCs obtained as in (A). A more rapid loss of fluorescence in the cumulus cells following bleaching of the oocyte indicates more extensive gap junctional coupling. (C) COCs were recovered from antral follicles of young (3-month) or aged (13-month) mice. The indicated mRNAs were quantified in the granulosa cells relative to Actb. Total number of oocytes in (A) was 32 (3-month) and 26 (13-month). See also Figure S3.