Figure 1. Pioglitazone inhibits high fat diet induced periprostatic white adipose inflammation.

A, study schema. C57BL/6J male mice were treated with low fat (LF) or high fat (HF) diets beginning at 6 weeks of age. After 12 weeks of feeding, two groups of mice fed either LF or HF diets were sacrificed. Two additional groups continued to receive LF diet or HF diet for an additional 8 weeks prior to sacrifice. Other mice received the HF diet for 12 weeks before being switched to HF diet containing either 0.006% or 0.06% w/w pioglitazone for an additional 8 weeks until sacrifice at 26 weeks of age. B, hematoxylin and eosin-stained section showing several CLS (arrows) in periprostatic fat. C, body weights of mice in different treatment groups. D, caloric consumption was monitored weekly. E, periprostatic white adipose inflammation defined as CLS/cm² was quantified at the time of sacrifice. Mean ± SD (error bars) are shown. D, n=9–10/group. E, n=4–10/group; ***p<0.001 compared with HF diet 20 weeks.