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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Cancer Prev Res (Phila). 2017 Dec 8;11(4):215–226. doi: 10.1158/1940-6207.CAPR-17-0296

Figure 2. Pioglitazone suppresses levels of proinflammatory mediators in periprostatic white adipose tissue in mice fed a high fat diet.

Figure 2

At 6 weeks of age, C57BL/6J male mice were fed either low fat (LF) diet or high fat (HF) diet. The LF diet fed mice were randomized to one of two groups. One group was sacrificed at 18 weeks of age after 12 weeks on LF diet while the second group was sacrificed at 26 weeks of age after 20 weeks on LF diet. The other mice were fed HF diet for 12 weeks to induce obesity and then randomized to one of four groups. One group was sacrificed at 18 weeks of age after 12 weeks on HF diet. The second group was continued on HF diet, the third group was switched to HF diet containing 0.006% w/w pioglitazone and the fourth group was switched to HF diet containing 0.06% w/w pioglitazone for an additional 8 weeks until sacrifice at 26 weeks of age. A, average adipocyte diameter. B–E, Real-time PCR was carried out for CD68, MCP-1, TNF-α and TGF-β. Mean ± SD (error bars) are shown. A, n=4–10/group; **p<0.01 compared with LF diet 20 weeks and ***p<0.001 compared with LF diet 12 weeks. B–E, n=3–7/group; **p<0.01 compared with HF diet 20 weeks.