Fig. 3.

CYP2E1 mRNA expression mediated by PhIP in breast cells. CYP2E1 expression in MCF-7 and MDA-MB-231 cells (a) was measured by RT-qPCR. The involvement of miR378 and JAK/STAT3 pathway in MCF-7 cells treated with different concentrations of PhIP was assessed by qPCR (b). Data were normalized to expression of GAPDH and are shown relative to control 0.1% DMSO. Correlation of mRNA expression of CYP2E1 and STAT3 in PhIP-treated MCF-7 cells is shown in (c). Statistical significance was calculated by linear regression in GraphPad Prism 6, ***p < 0.001. Error bars represent SEM for independent cultures (n = 3)