Fig. 6.

Increase in CYP2E1 expression and ROS generation by co-treatment of PhIP and ethanol: MCF-7 cells treated with ethanol (a) or PhIP 100 µM and ethanol 50 mM (b) for 24 h and CYP2E1 expression was determined by qPCR. Increase in ROS generation by co-treatment of PhIP and ethanol (c) in MCF-7 cells. Data is shown relative to control 0.1% DMSO. Statistically significant differences were calculated using one-way ANOVA with a Dunnett’s post test in GraphPad Prism 6, ***p < 0.0001–0.001, **p < 0.001–0.01. Error bars represent standard error of the mean (SEM) for independent cultures (n = 3)