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. 2018 Apr 3;8:5509. doi: 10.1038/s41598-018-23831-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Minimal inhibitory concentration (MIC) of DMAHDM, DMADDM and CHX dissolved in culture medium against planktonic S. mutans (mean ± SD; n = 3). MIC is the lowest concentration of an antimicrobial agent at which bacterial growth is completely inhibited. MIC was measured from passages 0 to 20. Each subsequent passage used the surviving bacteria from the previous passage that were exposed to the antibacterial agent, in order to test drug resistance. DMAHDM, DMADDM and CHX induced no drug resistance in S. mutans in 20 passages.