A paper-based point-of-care testing device that enables simultaneous colorimetric detection of multiple analytes. (a) The paper-based device can be designed to accommodate eight reaction zones for detection of eight different analytes and properties—leucocytes (LEU), nitrite (NIT), protein (PRO), pH, blood (BLO), specific gravity (SG), ketone (KET) and glucose (GLU). (b) Changes in colour were observed in the reaction zones for detection of glucose and protein (circled in red) after the addition of a test solution containing glucose and albumin. (c) Colour changes at the reaction zones can be compared to the colour chart from the manufacturer of the reagents strips for urinalysis, to determine the quantities of the various analytes present.