Fig. 6.

mOP-LTD of glutamate release at cholinergic interneuron synapses is not affected by EtOH. a Schematic figure of the injection paradigm enabling optogenetic recording from ChATCre+ DLS MSNs. A cre-recombinase-dependent AAV vector coding for ChR2 (AAV.DIO.ChR2) was injected 14 days prior to saline and EtOH treatment. b Coronal brain slice showing the infection of cholinergic interneurons in the DS (bar scale = 500 μm). c Representative light-evoked synaptic traces from the DLS before and after DAMGO (0.3 μM, 5 min) application in saline (blue traces) and EtOH (red traces)-injected mice. d, e EtOH was unable to disrupt mOP-LTD at cholinergic interneuron synapses in the DLS (P = 0.42, t₁₄ = 0.83, saline n = 7 from 2 mice and EtOH n = 9 from 4 mice). Unpaired Student’s t test. Data represent mean ± SEM