Fig. 7.

MORs from cortical inputs are necessary to induce mOP-LTD in the DLS. a Quantitative PCR analysis showing that the deletion of MOR in the frontal cortex of MOR-flox/Emx1Cre+ mice reduces MOR mRNA expression (Cre−: 1.01 ± 0.070; Cre+: 0.34 ± 0.044; P = 0.0013, t₄ = 8.113). Measurable MOR signal in Cre+ mice likely reflects expression in interneurons. b Quantitative PCR showing intact MOR expression in the thalamus of MOR-flox/Emx1Cre+ mice (Cre−: 1.01 ± 0.10; Cre+ : 1.21 ± 0.23; P = 0.5151, t₅ = 0.7002). c The elimination of MORs from cortical afferents in MOR-flox/Emx1Cre mice prevented mOP-LTD in DLS (P = 0.0025, t₁₀ = 4.006, n = 6 each, from 2 MOR-flox/Emx1Cre− mice and 3 MOR-flox/Emx1Cre+ mice). RQ relative quantification. Unpaired Student’s t test. **P < 0.01. Data represent mean ± SEM