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. 2018 Mar 28;9:624. doi: 10.3389/fimmu.2018.00624

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Copyright © 2018 Workman, Maina, Brooks, Kohanski, Cowart, Mansfield, Kennedy, Palmer, Adappa, Reed, Lee and Cohen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Air–liquid interface (ALI)’s with a genotype homozygous for the functional form of the T2R38 receptor (PAV/PAV) or a genotype homozygous for the non-functional form of the receptor (AVI/AVI) demonstrated no significant differences in 4-amino-5-methylamino-2′,7′-difluorescein (DAF-FM) fluorescence intensity in response to 0.1% quinine over a period of 7 min (n = 6 cultures per condition). (A) Nitric oxide (NO) release over a period of 5 min, and (B) maximal NO release during that same period.