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. 2018 Apr 3;8:5426. doi: 10.1038/s41598-018-23706-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phagocytosis of circulating extracellular vesicles by THP-1 monocytic cells. (A) Blood plasma 12.5 K fraction of EVs were labelled with PKH dye and their phagocytosis by THP-1 cells was quantified by flow cytometry. (B) Exofacial expression level of CD47 on EVs. (C) Representative dot plots of CD47 expression (D) Expression level of phosphatidylserine on circulating EVs. (E) Representative dot plots of Annexin V positive vesicles. The statistical analysis is based on two-sided Student’s t-test. ***p < 0.001, **p < 0.01, *p < 0.05. All data presented is expressed as mean ± SEM.