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. 2018 Apr 3;8:5568. doi: 10.1038/s41598-018-23929-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LIPUS pretreatment induced the protein expression of BDNF in left hemispheres of mice after MCAO. Mice were pretreatment LIPUS for 5 consecutive days (15 min daily) before MCAO procedure. The protein expressions of BDNF in both right and left cerebral hemispheres after MCAO on 7 day was determined by immunohistochemical staining (A). The BDNF-positive cells in hippocampal CA1 regions were counted (B). Data are presented as means ± SEM (n = 6 per group). *P < 0.05 sham group versus MCAO group; ^#P < 0.05, MCAO group versus MCAO + LIPUS group. S: sham, M: MCAO, M + U: MCAO + LIPUS.