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. 2018 Mar 28;9:313. doi: 10.3389/fphys.2018.00313

Figure 3.

Figure 3

Involvement of TLR2, TLR4, and RAGE in HMGB1-induced NLRP3 inflammasome expression in VSMCs. (A–C) VSMCs were transfected with siRNA for TLR2, TLR4, and RAGE, and knockdown efficacies were determined by Western blot using β-actin as an internal control. Data are expressed as means ± SEMs of duplicates pooled from 3 independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control (untreated cells). (D–F) VSMCs were transfected with siRNA (200 nM) for TLR2, TLR4, and RAGE, and then stimulated with HMGB1 for 24 h. The expressions of NLRP3, ASC, and Caspase-1 were determined by Western blot using β-actin as an internal control. Data are expressed as means ± SEMs of duplicates pooled from 4 to 6 independent experiments. *P < 0.05, **P < 0.01 vs. corresponding value in untreated cells. #P < 0.05 and ##P < 0.01 vs. corresponding value in negative control.