Figure 5.

The effect of sodium nitrite administration on ischaemia and reperfusion-induced (A) superoxide and (B) 3-nitrotyrosine (3-NT) productions, The superoxide production was determined in 5 sham control (SC), ischaemic control (IC) and nitrite treated (NaNO₂+I/R) dogs. For each n, 4 images were evaluated and averaged. The formation of 3-NT was assessed in four dogs in each group. Values for each n were calculated from three replicates. The representative images of the DHE staining and western blot with the corresponding Coomassie Blue staining are shown on the upper parts of the figure. Compared with the sham controls, occlusion and reperfusion of the LAD resulted in a marked increase in both the superoxide and 3-NT production. These changes were significantly less marked in dogs treated with nitrite 24 h previously and subjected to a similar period of I/R. Values are means ± S.E.M. ^*P < 0.05 compared with SC; ^#P < 0.05 compared with IC.