Table 1.
Statistical summary of the sequencing (via PacBio) and assembly of the sugarcane BAC pools from the Brazilian hybrid sugarcane cultivar SP80-3280.
PacBio sequencing | Celera assembly | ||||||||
---|---|---|---|---|---|---|---|---|---|
Name | N. BACs | Trimmed reads | E. coli % | Contigs | Longest contig | Smallest contig | Contig total length | N50 | GC (%) |
Pool 01 | 4 | 178,758 | 5 | 16 | 143,471 | 9,202 | 582,340 | 62,347 | 43.77 |
Pool 02 | 8 | 202,770 | 7 | 17 | 134,154 | 8,615 | 1,034,115 | 109,126 | 45.08 |
Pool 03 | 8 | 139,394 | 13 | 25 | 142,211 | 8,101 | 800,349 | 54,726 | 45.45 |
Pool 04 | 8 | 206,601 | 8 | 27 | 122,448 | 9,303 | 882,224 | 41,554 | 44.06 |
Pool 05 | 8 | 189,764 | 9.6 | 16 | 175,157 | 8,050 | 1,186,577 | 132,868 | 45.28 |
Pool 06 | 8 | 237,520 | 9 | 21 | 168,704 | 8,289 | 920,150 | 86,198 | 44.75 |
Pool 07 | 8 | 143,827 | 6.8 | 19 | 164,848 | 10,668 | 1,140,957 | 128,641 | 45.41 |
Pool 08 | 8 | 186,873 | 7.4 | 19 | 143,661 | 10,202 | 1,129,862 | 108,955 | 44.41 |
Pool 09 | 8 | 174,835 | 8.4 | 20 | 187,285 | 10,664 | 1,261,846 | 99,030 | 44.43 |
Total | 68 | 1,660,342 | – | 180 | – | – | 8,938,420 | – | – |