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. 2018 Mar 28;11:90. doi: 10.3389/fnmol.2018.00090

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Copyright © 2018 Benoit, Doubi-Kadmiri, Benigni, Crepin, Riffault, Poizat, Vacher, Taouis, Baroin-Tourancheau and Amar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ARC molecular characterization. The ARC harbors a neuronal population specifically expressing POMC transcripts when compared to the surrounding hypothalamic nuclei including the PVN. GAPDH and POMC expression were analyzed by RT-qPCR. POMC expression was normalized to GAPDH expression in each ARC and PVN. Ratio in one ARC of Group C-C (blue bar) was taken as the reference ratio. Ratios are shown using a log₁₀ scale. All ARCs displayed POMC to GAPDH ratios at least a hundred-fold higher than those characterizing the PVNs except one ARC of Group HF-C (hatched bar) that was excluded from further analyses. Twenty-four ARCs (six ARCs/group) were conserved for analyses of miRNA populations.