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. 2018 Apr 3;8:5521. doi: 10.1038/s41598-018-23780-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of AMPKα inhibition on TRAIL-BBR-induced apoptosis. (A) C42 cells overexpressing empty vector (C42-EV) or FLAG epitope-tagged dominant negative AMPKα1 vector (C42-DN) were treated with DMSO or TRAIL alone or TRAIL in combination with increasing concentrations of BBR for 8 hrs. The samples were analyzed by Western blot analyses with the indicated antibodies. (B) C42 cells were transiently transfected with 50 nM of either control siRNA (lanes 1 & 2), or AMPKα1 siRNA (lanes 3 & 4) or AMPKα2 siRNA (lanes 5 & 6) for 72 hrs and then treated with DMSO or a combination of TRAIL and BBR for 8 hrs. Western blot analysis was performed utilizing the antibodies indicated. For all western blots (A,B), the bands from adjacent lanes were cropped from the same blot (full-length uncropped blots are included in Supplementary Fig. S6).