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. 2018 Apr 3;8:5510. doi: 10.1038/s41598-018-22852-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The positive correlation between TRPM7 protein, supernatant LDH and cellular HMGB1 in HR TCMK-1 cells. Cytotoxicity was assessed by LDH release in TCMK-1 cells exposed to 12-h H and followed by 6-h, 12-h, and 24-h R (a). The positive correlation between TRPM7 expression and LDH in TCMK-1 cells (b). The expression of HMGB1 was also detected by western blotting in HR TCMK-1 cells (c). The positive correlation between TRPM7 and HMGB1 protein was revealed in TCMK-1 cells (n = 6, (e)).