Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 3;8:5580. doi: 10.1038/s41598-018-23785-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Image processing and quantitative analysis. Automatic cell counting adapted from Image J Plugins (a) Overlay of the identified seeds (yellow) with tdTomato + cells. Images were presented using maximum intensity projection. (b) Validation of automatic seed identification against 50 manually identified cells. (c) The counting accuracy of 5.7% ± 3.8% in the EGFP group, and 4.4% ± 3.5% in the tdTomato group was validated by 28 randomly picked regions (a total of 7127 and 9025 cells, respectively). Error bars represent standard deviation.