Table 2.
Thermodynamic parameters of binding of apoA-I 1‒83/G26R to SUVs at 25 °Ca.
| SUV | Kd (μg/ml) | Bmax (amino acids/mol PL) | ΔGb (kcal/mol) | ΔH (kcal/mol) | TΔSc (kcal/mol) |
|---|---|---|---|---|---|
| PC | 1.1 ± 0.5 | 0.14 ± 0.03 | −11.9 ± 0.3 | −20.3 ± 0.8 | −8.4 ± 0.8 |
| PC/Chol (2/1) | 3.8 ± 0.4 | 0.12 ± 0.02 | −11.1 ± 0.1 | −24.6 ± 0.1 | −13.5 ± 0.1 |
| PC/PS (17/3) | 1.0 ± 0.9 | 0.28 ± 0.07 | −12.1 ± 0.6 | −12.4 ± 0.1 | −0.3 ± 0.6 |
| PC/PS (7/3) | 0.5 ± 0.4 | 0.53 ± 0.19 | −11.6 ± 0.1 | −11.6 ± 0.1 | 0.9 ± 0.5 |
aThe data were from three independent experiments.
bFree energy was calculated according to ΔG = −RT ln 55.5(1/Kd).
cThe entropy of binding was calculated from ΔG = ΔH − TΔS.