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. 2018 Apr 3;8:5435. doi: 10.1038/s41598-018-23810-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viral vector-mediated reporter gene expression in striatum. (A) Confocal image of brain sections stained with antibodies against Cre (magenta) and GFP (Green). The ROSA26/CAG-floxed STOP-ChRFR(C167A)-Venus BAC actuator/reporter rat was injected with AAV2-Cre in its striatum. (B) Localization of Cre-immunoreactive nuclei (magenta) and Venus immunoreactivity (green) in neurons at striatum. (C) Orthogonal view on the brain image stack of a neuron which has Cre-positive nucleus. Note that Venus accumulated in the plasma membrane and did not merge with the Cre signals.