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. 2018 Apr 3;8:5575. doi: 10.1038/s41598-018-23881-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IFN-γ- and TGF-ß1-induced monocyte migration. (A) Flow cytometry analysis of CD11b⁺CD115⁺Ly6C⁺ stained cells shows differences between the ratios of Ly6C^low/Ly6C^high monocytes versus the different conditioned media in C4 fibroblasts. (B) Indication for enhanced migration of Ly6C^low monocytes towards TGF-ß1-conditioned media of cardiac fibroblasts, whereas co-stimulation with TGF-β1 + IFN-γ increases the number of migrated Ly6C^high monocytes. (C) Gating strategy to define the amount of CD11b⁺CD115⁺Ly6C⁺ cells. (D) Representative dot blots for the migrated monocytes of the different conditioned media of stimulated C4 fibroblasts (n = 6/condition). (E) Representative dot blots for the migrated monocytes of the different conditioned media of stimulated cardiac fibroblasts (n = 3/condition). Data are shown as ratio of Ly6C^low/Ly6C^high monocytes of 3 independent experiments. Statistical analysis was performed by One way-ANOVA or Kruskal Wallis test (^#p < 0.05 vs co, *p < 0.05 vs TGF-β1, ^§p < 0.05 vs IFN-γ).