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. 2018 Apr 3;8:5508. doi: 10.1038/s41598-018-23777-6

Figure 5.

Figure 5

IL-4 depletion does not impair post-immunization hapten-specific B cell early expansion, increases peptide-specific T cell differentiation, and enhances efficacy of a tetanus-diphtheria-pertussis vaccine. (A,B) BALB/c mice immunized once with KLH (s.c., n = 4), OXY-KLH (s.c., n = 20), and OXY-KLH (s.c.) plus αIL-4 mAb (i.p, n = 20). At 7 days after immunization, analysis of OXY-specific B cells in pooled lymph nodes and spleen by flow cytometry: (A) Antibody secreting cells, and (B) GC B cells. Data shown are from three experiments. (C–F) C57Bl/6 naïve mice (n = 5) and mice immunized once with PE-SA2W (n = 8) and PE-SA2W plus αIL-4 mAb (n = 8). At 7 days after immunization, analysis of 2W-specific CD4+ T cells in pooled lymph nodes and spleen by flow cytometry: (C) non-Tfh cells, (D) Tfh cells, (E) GC-Tfh cells, (F) (Tfh + GC- Tfh)/non-Tfh. Data shown are from two experiments. (G–I) Mice immunized with TDaP and TDaP plus αIL-4 mAb (n = 10 each group). (G) TT-specific IgG titer over time, (H) TT-specific IgG1 and IgG2a titers on day 28 after the first immunization, and (I) subset (%) of subjects showing anti-diphtheria protective antibodies. Data shown are from one experiment. Data are mean ± SEM. (A–E) One-way ANOVA paired with Tukey’s multiple comparisons test. (F and H) unpaired two tailed t-test. (G) Two-way ANOVA paired with Tukey’s multiple comparisons test. (I) Chi-square (χ2 = 52.43, df = 1). *p < 0.05, **p < 0.01 versus control, or brackets to indicate specific group differences.