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. 2018 Apr 3;8:5452. doi: 10.1038/s41598-018-23740-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of methods to detect P. lessonae over the sampling period from mid-May to end of August 2016. Left) observed and predicted (dashed line) rate of observation with eDNA (grey circle) and traditional methods (white circle). Grey bar (±S.E.) represent the average water temperature. n = number of samples (observational method in parenthesis). Right) Log odds ratios (±S.E.) for observation with the eDNA vs. the traditional methods. ***Indicate a p-value < 0.001.