Table 1.

In silico designed primers for Pelophylax lessonae tested in this study.

Primer ID	Sequence		Position	Annealing
cytB_15_F#	ATCGCCCAAATCGCAACAGG	forward	15	59.3
cytB_142_R#	GAAGAAGGATGCGCCGTTGG	reverse	142	61.3
cytB_56_F#	CACAGCTGACACATCCCTTG	forward	50	59.2
cytB_206_R#	GCCGTAATATAGGCCTCGTC	reverse	206	59.2
cytC1_64_F*	GGTGCATGAGCCGGGATAGT	forward	64	61.3
cytC1_166_R*	AAGGCGTGGGCGGTAACAAT	reverse	166	59.2
cytC1_858_F*	ATGGGCTCATCACATGTTCA	forward	858	59.2
cytC1_1007_R*	GGGGCTTCCCATTTAATGAT	reverse	1007	59.2
cytC1_676_F*	GACCCCGTTCTCTACCAACA	forward	676	59.2
cytC1_850_R*	ATCCCAGAAGGCCGATAGAT	reverse	850	57.2

^#Primers for the mitochondrial DNA (mtDNA) cytochrome b (database entry Q94RW7_PELLE).

*Primers for cytochrome c1 (database entry G8HSZ6_PELLE).