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. 2018 Apr 3;8:5420. doi: 10.1038/s41598-018-23833-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of the conditioned medium on TMD and BMD spheroids. Of note, MC = MC3T3 osteoblast-like cells, A5 = MLO-A5 osteocyte-like cells, and Y4 = MLO-Y4 osteocyte-like cells. The asterisk indicates statistical significance at p < 0.05. (A) Primary cell spheroids formed in media conditioned with MLO-A5 and MLO-Y4 spheroids after 24 h. (B) Roughness and area of the primary cell spheroids. (C & D) TMD and BMD spheroids in the conditioned media isolated from MC3T3, MLO-A5, and MLO-Y4 cultures. (E & F) Relative size change of TMD and BMD spheroids in the conditioned media.