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. 2018 Apr 3;8:5420. doi: 10.1038/s41598-018-23833-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Three-dimensional characterization of tumor-osteocyte interactions. (A & B) TMD and BMD cell spheroids (green), positioned adjacent to MLO-A5 spheroids (black) 48 h after 3D bioprinting. (C) Average fluorescence intensity along the axis perpendicular to the supporting needle shaft. (D & E) Click-iT EdU assay of proliferating cells in 4T1.2 cell spheroids and TMD cell spheroids, respectively, in the presence and absence of MLO-A5-conditioned media.