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. 2018 Apr 3;8:5420. doi: 10.1038/s41598-018-23833-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agarose bead assay. (A) Images of the edge of the agarose beads showing migration of TMD tumor cells under the beads loaded with various bone matrix proteins and compounds. (B) Time response of TMD cells migrating under control and collagen-loaded agarose beads. (C) Images of the edge of agarose beads in response to increasing concentrations of collagen. (D) Cumulative fraction of cell migration at the agarose bead edge for collagen, biglycan, and collagen biglycan combination. (E) Relative spheroid size correlates with relative bead migration by treatment with bone matrix proteins.