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. 2018 Apr 3;8:5424. doi: 10.1038/s41598-018-23631-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–C) Absolute capillary length (A), capillary volume (B) and capillary surface area (C) in 40, 60, 87-week-old KO and WT mice (*p < 0.05, **p < 0.01, ***p < 0.001) as determined by stereology. KO raw data; WT raw data; KO median; WT median; highest value still within 1.5 times the interquartile range of the third quartile of KO; highest value still within 1.5 times the interquartile range of the third quartile of WT; lowest value still within 1.5 times the interquartile range of the first quartile of KO; lowest value still within 1.5 times the interquartile range of the first quartile of WT; values between first and third quartile of KO data (indicating the middle 50% and the interquartile range); values between first and third quartile of WT data (indicating the middle 50% and the interquartile range); ↓ Capillaries; ӿ seminiferous tubules showing disturbed spermatogenesis. (D) Representative semithin sections from WT and KO testes of the different age groups are shown as an overview. (Arrows indicate examples of capillaries, asterisks mark tubules with disturbed spermatogenesis).

Inline graphic — (A–C) Absolute capillary length (A), capillary volume (B) and capillary surface area (C) in 40, 60, 87-week-old KO and WT mice (*p < 0.05, **p < 0.01, ***p < 0.001) as determined by stereology. KO raw data; WT raw data; KO median; WT median; highest value still within 1.5 times the interquartile range of the third quartile of KO; highest value still within 1.5 times the interquartile range of the third quartile of WT; lowest value still within 1.5 times the interquartile range of the first quartile of KO; lowest value still within 1.5 times the interquartile range of the first quartile of WT; values between first and third quartile of KO data (indicating the middle 50% and the interquartile range); values between first and third quartile of WT data (indicating the middle 50% and the interquartile range); ↓ Capillaries; ӿ seminiferous tubules showing disturbed spermatogenesis. (D) Representative semithin sections from WT and KO testes of the different age groups are shown as an overview. (Arrows indicate examples of capillaries, asterisks mark tubules with disturbed spermatogenesis).