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. 2018 Apr 3;8:5550. doi: 10.1038/s41598-018-23858-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Swimming motility of P. putida in the presence/absence of ICE. P. putida strains with (strain 2737) and without ICEclc (strain 3227) were pregrown in MM with 3CBA or LB and tested on swimming plates (PG medium solidified with 0.3% agar). (A) Measurement of the mean colony ring diameter (±1 SD, n = 5) after 19 h incubation. Asterisks above bar diagrams indicate significance of difference (P < 0.01) in one-tailed t tests. (B) Representative images of different colony sizes on swimming plates after 19 h of incubation of P. putida strains precultured in MM with 3CBA (upper panel) or in LB (lower panel).