Figure 5.

Differentiation of CD9-positive cells into endothelial cells. (A) Bright field images of primary cultured CD9-positive cells cultured for 120 h on laminin-coated surfaces in medium with 0.1% BSA (left) or 10% FBS (right) at 2.0 × 10⁴ cells/cm². (B) Immunocytochemistry of VE-cadherin (left, green) and in situ hybridisation of Kdr (right, arrowhead) after cultivation of CD9-positive cells for 120 h with 10% FBS. (C) Double-staining of isolectin B4 with CD9 (upper row), S100β (middle row), and SOX2 (lower row) in rat CD9-positive cells after primary culture for 120 h with 10% FBS. (D) Relative ratio of mRNA levels of the following genes after primary culture of CD9-positive cells with 10% FBS (white bar) or 0.1% BSA (black bar) as determined by qPCR (mean ± SEM, n = 3), followed by normalisation with an internal control (Actb): Left graph: Cd9, S100β, Sox2, Prop1, Cadh1, and Cxcr4. Right graph: Id2, Sox18, Nrp1, Kdr, Pecam1, and Edn.