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. 2018 Apr 3;8:5518. doi: 10.1038/s41598-018-23749-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ancestral gene linkages remain between a few pharyngeal cluster genes in tunicate genomes. Schematic depicting the organization of the pharyngeal cluster genes among the studied chordate genomes. Double-parallel lines indicate >1 Mb distance between genes. Chromosome (chr) or scaffold (S) number is shown, along with gene ID when available for newly annotated genomes. Orthologous genes are indicated by common colours. Transcript IDs for B. leachii genes identified in our transcriptome data³⁷ are provided in File S5.