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. 2018 Apr 3;8:5409. doi: 10.1038/s41598-018-23702-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decellularization produces acellular scaffolds with preserved ECM structure. Decellularized lung slices (Decell) compared to native lung slices (Native). (A) HE staining indicating intact morphology and complete removal of cell nuclei (lilac) after decellularization. (B) Elastic Van Gieson (EVG) staining with elastic fibers in dark blue. (C) Immunofluorescence staining of the basement membrane protein Collagen type IV (red) with nuclear DAPI counterstain (blue). (D) Scanning electron microscope (SEM) images of alveolar tissue. Scale bars: 50 µm. (E) DNA quantification of native and decellularized lung tissue, error bars show SD. (F) Relative distribution of summed protein intensities for respective matrisome or non-matrisome protein group.