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. 2018 Apr 3;8:5477. doi: 10.1038/s41598-018-23899-x

Figure 1.

Figure 1

Sertoli cells support high levels of ZIKV replication. (A) Primary human Sertoli and Leydig cells and the human A549 cell line were infected with ZIKV strain MR766 (MR) or PRVABC59 (PR) (MOI = 5) for 24, 48, 72 and 96 hours. At each time point, supernatants were harvested and viral titers were determined by plaque assay. (B–E) Cells were harvested at each time point and viral replication (B), Interferon-β (C), Interferon-λ2 (D) and OAS2 (E) mRNA levels were determined by qRT-PCR. (F) Sertoli cells seeded on coverslips were infected with ZIKV MR766 (MR) or PRVABC59 (PR) (MOI = 5) for 48 hours and then processed for indirect immunofluorescence using antibodies to ZIKV NS1 and GATA4 to identify infected cells expressing Sertoli markers. Nuclei were stained with DAPI. Representative images are shown. All values are expressed as mean ± standard error. N = 3.