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. 2018 Apr 3;8:5477. doi: 10.1038/s41598-018-23899-x

Figure 2.

Figure 2

Zika virus entry in Sertoli cells. Sertoli cells were pre-treated with antibodies to the indicated proteins (10 µg/ml) (A) or Axl inhibitor (R428) (B,D) for two hours and then infected with ZIKV PRVABC59 (PR) (A,B) or MR766 (MR) (D) (MOI = 1) for 48 hours after which total RNA was extracted and virus replication determined by qRT-PCR. ZIKV inocula ZIKV PRVABC59 (C) or MR766 (MR) (E) were incubated with indicated concentrations of duramycin at 37 °C for two hours and then used to infect Sertoli cells (MOI = 1) for 48 hours after which total RNA was extracted and virus replication determined by qRT-PCR. All values are expressed as mean ± standard error. *P < 0.05, **P < 0.01, **P < 0.001 (One-way ANOVA); N = 3.