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. 2018 Apr 3;8:5422. doi: 10.1038/s41598-018-23651-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High expression of B2M in MSCs and MSC^shB2M retained the multipotent differentiation ability of MSCs. (a) MSCs have a high expression of B2M while esophageal cancer cells (Eca109 and TE-1) barely express B2M, both at the mRNA (qRT-PCR; left panel) and at the protein (Western blot; right panel) level. (b) Construction of control RNAi (MSC^NTC) and B2M RNAi knockdown (MSC^shB2M) MSC cell lines, showing over 79% B2M knocking down effect by B2M RNAi, at both the mRNA (qRT-PCR; left panel) and the protein (Western blot; right panel) level. (c) The results of the flow cytometry analysis revealed that the MSC^NTC and the MSC^shB2M cells shared specific surface markers with normal MSCs (CD29, CD44, CD73 and CD105). (d) Light microscopy images of MSC^shB2M cell samples assayed for adipogenic (Oil Red O staining), osteogenic (Alizarin Red S staining), and chondrogenic (toluidine-blue staining) differentiation (left, middle, and right panels, respectively). The imaging results revealed that the MSC^shB2M retained its multipotency. CON: original bone marrow MSCs; Scale bars: 100 μm.