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. 2018 Apr 3;8:5451. doi: 10.1038/s41598-018-23806-4

Figure 3.

Figure 3

Stress tolerance assay in yeast mutants of MG detoxification enzymes in salinity stress: BY4741 wild type yeast cells, GLYIKO, GLYIIKO and D-LDHKO mutant yeast cells containing empty vector pYES2 were grown in presence of salinity stress (A). These mutant cells were transformed with their respective mutant genes cloned in pYES2 vector and the complemented yeast cells were grown in presence of salinity stress and their growth was monitored along with wild type BY4741 cells (B). MG levels were estimated in all of these samples after 30 hours and contribution of these genes in detoxification of MG was compared (C).